Imaging mitochondria live or fixed muscle tissues
نویسندگان
چکیده
منابع مشابه
High resolution imaging of live mitochondria.
Classically, mitochondria have been studied by biochemical, genetic and electron microscopic approaches. In the last two decades, it became evident that mitochondria are highly dynamic organelles that are frequently dividing and fusing, changing size and shape and traveling long distances throughout the life of a cell. The study of the complex structural changes of mitochondria in vivo became p...
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The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal ...
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Cardiac mitochondria are complex highly organized cellular organelles, which play central roles not only in energy homeostasis but also in various biosynthetic, signaling, and cell death pathways. Moreover, mitochondria are highly dynamic organelles that continuously divide and fuse as well as move within the cell. Therefore, it is not surprising that multiple methodological approaches have bee...
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Monitoring gene expression is crucial for studying the responses of gene therapy and clarifying gene function in various environments. Molecular imaging is a powerful tool for noninvasive visualization of gene expression. This article summarizes the current status of fluorescence and bioluminescence imaging (BLI) of gene expression in live cells and tissues, with the emphasis mainly on the earl...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2018
ISSN: 2043-0116
DOI: 10.1038/protex.2018.101